Journal: Journal of Clinical Microbiology

Article Title: Limited Utility of Culture for Mycoplasma pneumoniae and Chlamydophila pneumoniae for Diagnosis of Respiratory Tract Infections ▿

doi: 10.1128/JCM.00321-10

Figure Lengend Snippet: Total numbers of samples tested and percentages positive by the various methods used for Mycoplasma pneumoniae and Chlamydophila pneumoniae diagnosis

Article Snippet: From 2003 to 2008, microbiology results of culture, PCR, and serology performed at ARUP Laboratories for M. pneumoniae and C. pneumoniae were retrospectively reviewed with a specific focus on respiratory specimens (e.g., nasal wash, nasopharyngeal swab, bronchoalveolar lavage, tracheal aspirate, sputum, and pleural fluid) for PCR and culture.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Enfermedades Infecciosas Y Microbiologia Clinica

Article Title: ADN de Chlamydia trachomatis en leucocitos de sangre periférica de neonatos

doi: 10.1016/j.eimc.2014.09.019

Figure Lengend Snippet: Características clínicas y microbiológicas de neonatos positivos y negativos a PCR para la detección de ADN de Chlamydia trachomatis

Article Snippet: La técnica de microinmunofluorescencia (MIF) se llevó a cabo de acuerdo con el procedimiento descrito en el instructivo de los proveedores ( Chlamydia pneumoniae IgG/IgM Micro-IF Test kit, Ani Labsystems Ltd. Oy, Vantaa, Finlandia).

Techniques:

Journal: Enfermedades Infecciosas Y Microbiologia Clinica

Article Title: ADN de Chlamydia trachomatis en leucocitos de sangre periférica de neonatos

doi: 10.1016/j.eimc.2014.09.019

Figure Lengend Snippet: Distribución del título de anticuerpos IgM por microinmunofluorescencia (MIF) contra pacientes positivos o negativos a PCR para Chlamydia trachomatis

Article Snippet: La técnica de microinmunofluorescencia (MIF) se llevó a cabo de acuerdo con el procedimiento descrito en el instructivo de los proveedores ( Chlamydia pneumoniae IgG/IgM Micro-IF Test kit, Ani Labsystems Ltd. Oy, Vantaa, Finlandia).

Techniques:

Journal: Enfermedades Infecciosas Y Microbiologia Clinica

Article Title: ADN de Chlamydia trachomatis en leucocitos de sangre periférica de neonatos

doi: 10.1016/j.eimc.2014.09.019

Figure Lengend Snippet: Sensibilidad y especificidad de los anticuerpos IgM anti- Chlamydia trachomatis en sueros de recién nacidos pretérmino. El método empleado fue microinmunofluorescencia.

Article Snippet: La técnica de microinmunofluorescencia (MIF) se llevó a cabo de acuerdo con el procedimiento descrito en el instructivo de los proveedores ( Chlamydia pneumoniae IgG/IgM Micro-IF Test kit, Ani Labsystems Ltd. Oy, Vantaa, Finlandia).

Techniques:

Journal: Enfermedades Infecciosas Y Microbiologia Clinica

Article Title: ADN de Chlamydia trachomatis en leucocitos de sangre periférica de neonatos

doi: 10.1016/j.eimc.2014.09.019

Figure Lengend Snippet: Sensibilidad y especificidad de las pruebas de diagnóstico para la detección de Chlamydia trachomatis

Article Snippet: La técnica de microinmunofluorescencia (MIF) se llevó a cabo de acuerdo con el procedimiento descrito en el instructivo de los proveedores ( Chlamydia pneumoniae IgG/IgM Micro-IF Test kit, Ani Labsystems Ltd. Oy, Vantaa, Finlandia).

Techniques:

Journal: PLOS Neglected Tropical Diseases

Article Title: Guillain-Barré syndrome after the Zika epidemic in Colombia: A multicenter, matched case-control study

doi: 10.1371/journal.pntd.0012898

Figure Lengend Snippet: Molecular and serological diagnosis of infectious disorders in GBS cases and controls.

Article Snippet: Assessment of M. pneumonie and hepatitis E virus (HEV) infection was based on testing of anti- M. pneumoniae IgM (cat. EI 2202-9601 M EUROIMMUN, Lübeck, Germany), anti-HEV IgM (cat. EI 2525-9601 M, EUROIMMUN, Lübeck, Germany); For HEV and M. pneumoniae a positive IgM was interpreted as a recent infection, and a negative IgM ruled out infection [ ].

Techniques: Biomarker Discovery, Virus, Infection

Journal: PLOS Neglected Tropical Diseases

Article Title: Guillain-Barré syndrome after the Zika epidemic in Colombia: A multicenter, matched case-control study

doi: 10.1371/journal.pntd.0012898

Figure Lengend Snippet: Association analysis of clinical characteristics and infection assessment with GBS.

Article Snippet: Assessment of M. pneumonie and hepatitis E virus (HEV) infection was based on testing of anti- M. pneumoniae IgM (cat. EI 2202-9601 M EUROIMMUN, Lübeck, Germany), anti-HEV IgM (cat. EI 2525-9601 M, EUROIMMUN, Lübeck, Germany); For HEV and M. pneumoniae a positive IgM was interpreted as a recent infection, and a negative IgM ruled out infection [ ].

Techniques: Infection, Virus

Journal: Transfusion

Article Title: Convalescent plasma treatment of critically ill intensive care COVID ‐19 patients

doi: 10.1111/trf.16392

Figure Lengend Snippet: SARS‐CoV‐2 IgG ELISA (Euroimmun) ratios in sera of convalescent volunteers and in control samples. In total, 61 sera from convalescent, potential blood donors with a SARS‐CoV‐2 PCR‐confirmed infection (left panel) and in twenty 3‐year‐old retention samples from our blood bank (right panel) were tested for SARS‐CoV‐2 spike S1 domain–specific IgG. Samples were classified as positive (OD Ratio ≥ 1.1), borderline (OD Ratio ≥ 0.8 to <1.1), or negative (OD Ratio < 0.8). Horizontal bars represent the median and the interquartile range. The gray shaded area indicates borderline results

Article Snippet: SARS‐CoV‐2–specific antibodies were determined by an enzyme‐linked immunosorbent assay detecting immunoglobulin G (IgG) antibodies against the S1 protein (Euroimmun), and the neutralizing titers were determined with a cell‐culture‐based neutralization assay.

Techniques: Enzyme-linked Immunosorbent Assay, Control, Infection

Journal: Transfusion

Article Title: Convalescent plasma treatment of critically ill intensive care COVID ‐19 patients

doi: 10.1111/trf.16392

Figure Lengend Snippet: Distribution of antibody ratios in sera of convalescent volunteers acquired at different periods after the disease onset. SARS‐CoV‐2 spike S1 antibodies were measured by ELISA (Euroimmun) in sera from convalescent, potential blood donors with PCR‐confirmed SARS‐CoV‐2 infection. Correlation analysis was performed by Spearman's test. The continuous line shows the regression line, and the broken lines show the 95% confidence interval

Article Snippet: SARS‐CoV‐2–specific antibodies were determined by an enzyme‐linked immunosorbent assay detecting immunoglobulin G (IgG) antibodies against the S1 protein (Euroimmun), and the neutralizing titers were determined with a cell‐culture‐based neutralization assay.

Techniques: Enzyme-linked Immunosorbent Assay, Infection

Journal: The Journal of Immunology Author Choice

Article Title: Identification of a Novel Keto Sugar Component in Streptococcus pneumoniae Serotype 12F Capsular Polysaccharide and Impact on Vaccine Immunogenicity

doi: 10.4049/jimmunol.2100849

Figure Lengend Snippet: S. pneumoniae serotype 12F polysaccharide structure. (A and B) The structure is based on previously published degradation and derivatization analysis (25) (A), and modern NMR and MS analysis of PFESP00919 12F polysaccharide proposed in the present study (B).

Article Snippet: Purified and lyophilized S. pneumoniae cell wall polysaccharide (C-Poly) was purchased from Cedarlane (supplied by Statens Serum Institut, København, Denmark).

Techniques:

Journal: The Journal of Immunology Author Choice

Article Title: Identification of a Novel Keto Sugar Component in Streptococcus pneumoniae Serotype 12F Capsular Polysaccharide and Impact on Vaccine Immunogenicity

doi: 10.4049/jimmunol.2100849

Figure Lengend Snippet: Prevalence of Sug substitution and genotypic characterization in S. pneumoniae serotype 12F isolates

Article Snippet: Purified and lyophilized S. pneumoniae cell wall polysaccharide (C-Poly) was purchased from Cedarlane (supplied by Statens Serum Institut, København, Denmark).

Techniques: Sequencing, Residue

Journal: The Journal of Immunology Author Choice

Article Title: Identification of a Novel Keto Sugar Component in Streptococcus pneumoniae Serotype 12F Capsular Polysaccharide and Impact on Vaccine Immunogenicity

doi: 10.4049/jimmunol.2100849

Figure Lengend Snippet: 12F immune sera are equally effective at killing the 12F pneumococcal isolates with Sug substitution levels between ∼1.9 and ∼25.2%. PCV13 and PCV20 vaccines belong to the PREVNAR series and contain CRM197-conjugated polysaccharides from 13 and 20 serotypes of S. pneumoniae. PPSV23 vaccine contains native polysaccharides from 23 serotypes of S. pneumoniae. Both PPSV23 and PCV20 contain the 12F polysaccharide Ag.

Article Snippet: Purified and lyophilized S. pneumoniae cell wall polysaccharide (C-Poly) was purchased from Cedarlane (supplied by Statens Serum Institut, København, Denmark).

Techniques: Vaccines